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KMID : 0903520090520020221
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
2009 Volume.52 No. 2 p.221 ~ p.226
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Biochemical Characterization of Collagen from the Starfish Asterias amurensis
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Lee Ka-Jeong
Park Hee-Yeon
Kim Yeon-Kye
Park Jin-Il
Yoon Ho-Dong
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Abstract
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The industrial use of bovine and porcine collagen has been expanded to include foods, cosmetics, and biomaterials. However, due to outbreaks of bovine spongiform encephalopathy and foot and mouth disease, new, alternative, safe sources of collagen are being sought. In this study, pepsin solubilized collagen (PSC) was isolated from the tissue of a starfish (Asterias amurensis) and shown to consist of (α1)2α2 heterotrimer, which is similar to calf skin type I collagen. However, the PSC was denaturated at 24.7 C, which is about 12 C lower than mammalian collagen. Immunoblotting assay using polyclonal anti-type I collagen antibody revealed that the starfish collagen contained similar affinity motifs. In XTT assay, PSC suspension had cell growth activity and no showed no cytotoxicity.
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KEYWORD
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characterization, collagen, immunoblotting assay, starfish, XTT assay
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